IN-VITRO ANTIMICROBIAL AND IN-VIVO CENTRAL NERVOUS SYSTEM - LOCOMOTOR ACTIVITIES OF SOME SYNTHESIZED 2- [(1-((PHENYL AMINO) METHYL) SUBSTITUTED 1- BENZOIMIDAZOL-2-YL) ALKYL] ISOINDOLINE-1, 3-DIONES
Some new two series of 1, 2-disubstituted benzimidazolyl isoindoline derivatives (6a-n) were synthesized by reaction of 2- substituted benzimidazolyl isoindolines (4a-b) with various aromatic primary amines (5a-g) with formaldehyde in acid as a condensing agent. The 2-(1,3-dioxoisoindolin-2-yl) carboxylic acids (3a-b) cyclised with 1,2 diamino benzene yields (4ab). The pthalic anhydride (1) and amino acids (2a-b) fused at 180° c-185° c to give (3a-b). The structures of the synthesized isoindolines were verified by spectral analysis. The synthesized isoindoline1, 3-diones were screened for their in-vitro antimicrobial activity against standard pathological bacterial and fungal strains. All the isoindolines, exhibited a MIC values ranging between 1.6 to 204.8 μg for tested bacterial and fungal species. The most of the synthesized isoindoline1, 3-diones are shows good activity against the tested fungal species and gram positive bacteria. The most of the isoindolines shows poor activity against gram negative bacteria. The isoindolines have better activity against the tested candida than Aspergillus and Penicillium fungal species. The isoindolines (4a-b) and (6a-n) were screened for their in-vivo central nervous system locomotor activities. Among these tested isoindolines 4a, 4b, 6a, 6b, 6e, 6f were only shows elevated central nervous system depressant potency and remaining isoindolines were depicted bad central nervous system depressant activity