IN VITRO ANTIOXIDANT ASSAY AND ANTICARCINOGENIC EFFECT OF SOLANUM TUBEROSUM IN HEPG2 CELL LINE
Autoxidation of polyunsaturated fatty acids not only lowers the nutritional value of food but is also associated with membrane damage, aging, heart diseases and cancer in living organisms. Solanum tuberosum was obtained from plant cultivator. It was authenticated at the National Institute of Herbal Science, Plant Anatomy Research Center Chennai as the tubers of potatoes and was confirmed as Solanum tuberosum under the family Solanaceae. The objective of this study was to determine the antioxidant activity of Solanum tuberosum using various assays and to identify its anticarcinogenic effect in HepG2 cell lines. Its antioxidant property was evaluated by six standard methods namely DPPH radical scavenging, FRAP, Nitric Oxide, Superoxide anion scavenging, hydrogen peroxide scavenging and Hydroxyl radical scavenging assay. The antioxidant compounds like BHT, ascorbic acid, Quercitin were used as standards. MTT assay was performed to estimate the optimum dosage for the treatment of hepatic carcinoma