GENETIC VARIATION OF 15 AUTOSOMAL SHORT TANDEM REPEAT (STR) LOCI IN THE DIYALA- IRAQI POPULATION
Allele frequencies for the 15 autosomal STR loci included in the AmpFlSTR1 IdentifilerTM PCR Amplification Kit panel from Applied Biosystems (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO, D19S433, D2S1338, D16S539) and several statistical parameters were estimated from a sample of 139 unrelated individuals. samples were extracted using a PrepFiler Forensic DNA Extraction Kit (Applied Biosystems, Foster City, CA). a different number of alleles were observed with frequencies ranging between 0.004 (D8S1197- allele 9 and 18 , D21S11- allele 26 and 33, D3S1358-allele 12,13 and 20 , D19S433-allele 18 , TPOX – allele 6and 7, D18S51 - allele 24 and FGA- allele 23.2) and 0.486 (TPOX-allele 8). The highest FIS value for Diyala population has been calculated as D16S539 ( 0.08) and low FIS value- D21S11 (-0.06). No significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed (a 5% significance level was taken) in the Diyala- Iraqi Population. The exceptions were the D21S11 (P-value =0.00050), D16S539 (P-value =0.0047642), D2S1338 (P- value =0.0218069) , D19S433 (p-value= 0.0161895) and FGA (P-value =<0.00001)