DEVELOPMENT AND VALIDATION OF HPLC FOR SIMULTANEOUS ESTIMATION OF SULBACTAM AND ROPENEM IN BULK AND IN COMBINED DOSAGE FORM
A simple, accurate and precise HPLC method have been developed and validated for simultaneous estimation of Meropenem and Sulbactam Sodium in bulk and injectable dosage form. In HPLC method for simultaneous estimation of Meropenem and Sulbactam Sodium separation was achieved on Phenomenex C18H column (250 ×4.6 mm i.d., 5 μm), optimum mobile phase consisted of ratio of Pottasium dihydrogen phosphate buffer (pH-3.5 adjusted with orthophosphoric acid): Acetonitrile (90:10). The mobile phase at a flow rate of 1.0 ml/min, Injection volume 20μl and detection wavelength was kept at 265 nm. The retention time Meropenem and Sulbactam Sodium was 5.793±0.1min and 4.623±0.1min, respectively. The proposed conditions were successfully applied for the simultaneous estimation of both drugs in commercial injectable dosage form.