e -Issn : 0976 - 3651
Print-Issn : 2229-7480

  ABSTRACT

THE ANTICONVULSANT ACTIVITY OF BONE MARROW-DERIVED MESENCHYMAL STEM CELLS IN PTZ-INDUCED EPILEPTIC SEIZURES

Cell therapy based on stem cells provides a promise and safe treatment for numerous of central nervous system (CNS) disorders, but their mechanisms of action are still understood. Therefore, the present study aimed to investigate the possible anticonvulsant activity of bone marrow-derived mesenchymal stem cells (BM-MSCs) in pentylenetetrazole (PTZ)-induced epileptic seizures. Sixty adult female albino rats were divided into four groups; the first served as control, the second was injected intraperitoneally with PTZ, the third group injected with PTZ then treated with BM-MSCs for 30 days and the fourth group treated with BM-MSCs for 60 days. The levels of glutamate, aspartate, gamma aminobutyric acid (GABA), glycine, malondialdehyde (MDA), nitrite/nitrate, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), estrogen, luteinizing hormone (LH), follicular stimulating hormone (FSH) and thyroxine (T3) have been investigated. The treatment of epileptic model with BM-MSCs for 30 and 60 days restored the imbalance between the excitatory (glutamate and aspartate) and the inhibitory amino acids (GABA and glycine) neurotransmitters in cerebral cortex, cerebellum and hippocampus. Additionally, they were able to ameliorate the alterations in the oxidative status, hormones profile and the cytokines levels, also, the histopathological examinations showed improvement in the degenerated tissues especially in cerebellum after 60 days. From these findings it could be concluded that the anticonvulsant activity of BM-MSCs might be due to their neuroprotective, antioxidant and the immunomodulatory effects

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