FORMULATION AND EVALUATION OF OFLOXACIN NANOPARTICLES BY EMULSION POLYMERIZATION METHOD
The goal of this study was to formulate and evaluate the ofloxacin nanoparticles to improve the therapeutic efficacy of ofloxacin by loading in nanoparticle drug delivery system. Nanoparticles are especially designed to release the drugs in the vicinity of the target tissue. Nanoparticles are solid colloidal particles with diameter ranging from 1nm – 1000nm.Ofloxacin is a first generation fluroquinolones antimicrobials it active against gram negative bacteria. Ofloxacin nanoparticles was prepared by emulsion polymerization method by using Eudragit S-100 polymer as a continuous phase for four different ratios such as (Drug: Polymer) (1:1), (1:2), (1:3) and (1:4).The polymer was dissolved in Dichloromethane and the solution was emulsified with the aqueous solution of ofloxacin containing 2% Tween 80 by stirring for 1 hrs at 15C with the aid of mechanical stirrer at 3000rpm.The nanoparticles was formed and separated by centrifugation. The formulated nanoparticles was evaluated for external morphological studies using scanning electron microscope, Drug content, invitro release studies and IR spectral analysis. The invitro release study was performed in phosphate buffer PH 7.4. In IR study reveals that more in no major shifting as well as non less of functional groups peak between the drug, polymer and drug loaded nanoparticles. The invitro release study showed that the formulation ratio (1:1) gives the better sustained effect over 88.13 % of drug was released at 8 hours. This study conclude that the ofloxacin nanoparticles are suitable candidates to produce good antibiotic prolong action of the drug nanoparticles.