EFFECT OF COMBINATION OF SUBEROYLANILIDE HYDROXAMIC ACID AND O2ʹ, O3ʹ, O5ʹ-TRI-ACETYL-N6-(3-HYDROXYLANILINE) ADENOSINE ON VARIOUS OBESITY RELATED MARKERS IN 3T3-L1 PRE-ADIPOCYTIC CELL LINE
Combinational therapies are among the attractive treatment strategies for the pathological states where multiple regulatory mechanisms contribute to the development and the progression of the disease. In our present study, we investigated the effects of combination of Suberoylanilide hydroxamic acid [SAHA] and O2', O3', O5' -tri-acetyl-N6-(3-hydroxylaniline) adenosine [THA] on viability, adipogenesis and apoptosis in 3T3-L1 pre-adipocytic cell line. Mature adipocytes when treated individually with either SAHA or THA decreased cell viability but the combination of SAHA and THA caused an enhanced decrease in cell viability. The combination of drugs also showed an enhanced inhibition of adipocyte differentiation as monitored by Oil Red O Staining. The western blot analysis revealed that SAHA and THA when used in combination decreased the expression levels of peroxisome proliferator-activated receptor gamma (PPAR-γ) and CCAAT/enhancer binding protein (C/EBPα), both of which act as key regulators of adipogenesis. Furthermore, the combination of drugs increased the expression levels of Bcl-2 associated X protein (Bax) which is a pro-apoptotic protein involved in mediating apoptosis. Thus, the combination of SAHA and THA seem to exert multi -potent anti-obesity effects and seem to have promising benefits for the treatment and prevention of obesity