EFFECT OF SIZE REDUCTION TECHNIQUES ON DOXORUBICIN HYDROCHLORIDE LOADED LIPOSOMES
Long-circulating liposomes such as PEG-liposomes are frequently studied for drug delivery and diagnostic purposes. The present investigation is deals with to study the effect of different size reduction techniques on the physicochemical properties and pharmacological action of Doxorubicin hydrochloride loaded liposomes. Doxorubicin hydrochloride loaded liposomes were prepared by a sonication method. The maximum encapsulation efficiency, appropriate particle size and poly dispersity index (PDI) of several formulations with different phospholipid composition were evaluated by pH gradient method. The entrapment efficiency of liposomes produced using extrusion was high as compared to homogenization technique. LD50 of DOL-E was estimated to be greater than 30 mg/kg while LD50 of DOL-H to be about 30 mg/kg, which revealed that the extrusion method has better entrapment efficiency then the homogenization technique. The liposomes produced using extrusion method for reducing the size takes longer time as compared with homogenization technique while on the other hand it has got better entrapment efficiency, better PDI (More close to 0), narrow particle size distribution and better uniformity of particles. Therefore the particles produced using extrusion method will have better circulation time inside the body and will be less toxic