DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE ESTIMATION OF SILODOSIN IN BULK AND PHARMACEUTICAL DOSAGE FORM
A simple and economical RP-HPLC method was developed for the estimation of Silodosin in bulk and tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a C18 column (250 mm× 4.6mm, 5µ) at ambient temperature. The mobile phase consists of Methanol, acetonitrile, Water in the ratio 40:40:20v/v. The UV detection wavelength was 269 nm. The retention time for silodosin was 2.5 min. The method was validated for several parameters such as accuracy, precision, robustness as per ICH guidelines. Linearity was observed in the range of 10-60 µg/ml with correlation coefficient of 0.9997.The mean recovery were found to be in the range of 98.9-100.2% and the % RSD for interday and intraday was found to be 0.56 and 0.73 respectively. The results of the study show that the proposed RP-HPLC method is simple, rapid, precise and accurate and hence can be successfully used for the routine analysis of Silodosin in bulk and pharmaceutical formulation