EVALUATION OF TOTAL IMMUNOGLOBULIN [IgG] IN THE TUBERCULOSIS PATIENTS
The study aimed was carried out to evaluate the total IgG in tuberculosis patients. Tuberculosis is a chronic, necrotizing infection caused by Mycobacterium tuberculosis. The clinical manifestations of disease are the results of a balance between the host response and bacterial virulence. Single Radial Immunodiffusion was adopted for the estimation of total IgG in 87 tuberculosis patients (N = 87) and compared with 42 controls (N = 42). (1%) Agar in Barbitone buffer and mixed with an equal volume of Rabbit Anti-Human IgG suitably diluted in the same buffer. The mixer was poured over the precoated glass plates. Accurately measured different conc. of Human IgG and samples were loaded in the wells and incubated in a humid atmosphere for 24 hrs. The best specificity and positive predictive values were obtained with the IgG assay. The results analyzed showed a slight increase of IgG when compared to the controls in relation to age and sex. The percentage frequency of male patients found to be 76.89%. The percentage frequency of female patients found to be 23.11%. In controls, the average IgG level was 12.56 mg/ml. In TB patients, there was a slight increase the IgG level found to be 14.4 mg/ml. Therefore, the study of IgG concentrations can be an important and its help to distinguish those populations.