MEMBRANE STABILIZING POTENTIAL OF ECBOLIUM VIRIDE ON ACETAMINOPHEN PROVOKED HEPATOTOXICITY
The present study investigated the preliminary phytochemical investigation and protective effect of ethanolic and aqueous extract of leaves of Ecbolium viride (Forsk.) Alston on acetaminophen (APAP) provoked hepatotoxicity in rats. Preliminary phytochemical analysis of EVEE and EVAE had showed the presence of alkaloids, carbohydrates, phenols, gums & mucilage, flavonoids, terpenes, steroids and proteins. Acute toxicity studies data of E.viride ethanolic extract (EVEE) and E. viride aqueous extract (EVAE) revealed the safety profile of extracts at 2000mg/kg. EVEE and EVAE was administered at the dose of (200 & 400 mg/kg p.o), for 14 days. Hepatotoxicity was induced by oral intubation of APAP at the dose of 2g/kg on 14th day of treatment protocol. APAP intoxication resulted in significant increase in the serum hepatic markers like aspartate transaminase, alanine transaminase, alkaline phosphatase, acid phosphatase, γ-glutamyl transpeptidase, lactate dehydrogenase and total bilirubin. Furthermore, APAP altered the hematological profiles like total leukocyte count, total erythrocyte count and hemoglobin content and lipid parameters. Treatment with EVEE and EVAE had significantly ameliorated the toxic events of APAP and maintained the structural integrity of the hepatocytes. In conclusion, Ecbolium viride may be useful as a potential candidate preventing hepatic damage induced by APAP