QUALITATIVE ANALYSIS OF SALIVA PROTEINS IN ORAL CANCER SEPARATED BY SDS-PAGE
This study was conducted for qualitative estimation of saliva proteins separated by SDS-PAGE in organizes to describe the definition of potential saliva proteins that may act as diagnostic marker in oral cancer. Saliva samples of biopsy confirmed cases of oral cancer and normal controls of similar age group were subjected to SDS-PAGE on a 15% resolving gel, followed by staining with Coomassie Brilliant Blue R-250. Protein fractions were analyzed using computer software program ―Gene Genius Gel Documentation and Analysis System‖. Major protein fractions ranging in molecular weights from 4.8-98 kDa were observed with different intensity. Raw volumes of most of the protein fractions seem to be increased in majority of oral cancer cases as compared to normal control. Protein fractions 69 and 96 kDa were undetected in normal controls but appeared in 66% of Oral cancer patient. Further advancement of this work could yield improved resolution of protein fraction 69 and 96 kDa that could serve as marker for oral cancer.