PLASMID PROFILING AND BLA GENE AMPLIFICATION FROM PSEUDOMONAS SPECIES ISOLATED FROM HOSPITALIZED PATIENTS
Pseudomonas aeruginosa were isolated from pus and urine sample of hospitalized patient and confirmed morphologically and biochemically. Two different pigment pyocycin (greenish colour) and pyoverdin (UV-fluorescing pigment) were analysed for photostability and its antibacterial activity. Two strains were sensitive to pyocin produced by urine isolate. Three strains of Pseudomonas aeruginosa were isolated from Urine, Pus sample. It is confirmed morphologically and biochemically. Pseudomonas aeruginosa was confirmed by its growth in Cetrimide agar (Inhibitor of all other Gram Positive and Gram Negative bacteria) and its pigment production. Different molecular sized plasmid were isolated and transformed in DH 5ï¡ cells. ï¢-Lactamase providing genes might be present in plasmids, and it was confirmed with plasmid isolation from transformed cells. The BLA gene coding beta lactamase having the size of 1 kb was amplified.