DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF OFLOXACIN AND CEFEXIME FROM COMBINED ORAL DOSAGE FORM
A Simple, accurate, sensitive, cost effective and specific spectrophotometric method was developed for the quantization estimation of Cefexime (CEF) and Ofloxacin (OFL) in solid dosage form. In simultaneous equation method both CEF and OFL shows maximum absorbance at 237.00 nm and 288.00 nm respectively in 0.1N NaOH. The linearity ranges for CEF and OFL was 10-50µg/ml. The percentage drug estimated in marketed preparation was found to be 99.61±0.1298 and 99.56±0.2371 for CEF and OFL. The method was validated for various parameters as per ICH guidelines and USP requirements. The accuracy of the method was assessed by recovery studies and was found to be 99.15 - 99.52% and 99.43 - 99.85% for CEF and OFL respectively. Therefore the proposed method can be used for routine analysis in quality control laboratories