APOPTOSIS INDUCED IN HEPATOMA CELLS (HepG2) BY ALLYL ISOTHIOCYANATE ASSOCIATED WITH EXPRESSION OF P53 AND ACTIVATION OF CASPASE-3
The present study was undertaken to evaluate the p53 and Caspase 3 gene expression potential of Allyl isothiocyanate on Human hepatoma cell line HepG2. The viability of HepG2 was significantly reduced by Allyl isothiocyanate. The lactate dehydrogenase (LDH) was significantly released from Allyl isothiocyanate (5µM and 10 µM) treated HepG2 cells. Reduced Glutathione (GSH) was significantly reduced in Allyl isothiocyanate (5µM and 10 µM) treated HepG2 cells. Significant morphological changes were observed in fluorescent microscopic study. It indicates the apoptosis induction by Allyl isothiocyanate (5µM and 10 µM) in HepG2 cells. Noticeable changes in the intensity of pro-apoptotic protein bands were observed in Allyl isothiocyanate treated HepG2 cells. Expression of p53 and active caspase 3 in Allyl isothiocyanate (5µM and 10 µM) treated cells suggest that apoptosis induced by AITC. In conclusion, these results suggest that Allyl isothiocyanate (5µM and 10 µM) possessing significant apoptotic property with expression of p53 and active caspase 3.