COMPARATIVE STUDIES OF ACIDIC BLUE - G DYE DECOLORIZATION BY ASPERGILLUS FLAVUS AND ASPERGILLUS FUMIGATUS FROM TEXTILE SLUDGE, DEGRADATION ASSESSED BY HPLC
The objective of this research work is to validate the ability of fungal isolates (Aspergillus flavus and Aspergillus fumigatus) from textile sludge to decolorize Acidic Blue - G. The prime selection of isolates was evaluated on solid medium containing 100mg/l of dyes for decolorization and clear zones were found around the colonies, maximum fungal growth (cm) was observed in Aspergillus flavus (7.33±1.30) diameter and Aspergillus fumigatus exhibited maximum decolorization (5.33±0.42). After screening, aqueous batch degradation studies were carried out with varying concentrations of dyes (20-100 mg/l) in C - limited medium, Aspergillus flavus and Aspergillus fumigatus showed 79.91% and 70.13% in Acidic Blue - G (20 mg/l). Overall Percentage of decolorization of Aspergillus flavus and Aspergillus fumigatus elucidates about 75.02%. UV-Vis spectrometric analysis showed the before and after decolorization, fungal treatment suggested that decolorization was due to biodegradation. Decolorized extort of fungal isolates was assessed by HPLC analysis. HPLC elution profile showed that retention time (RT) of 2.162 for Acidic Blue-G, Aspergillus flavus (RT at 2.4, 5.0) and Aspergillus fumigatus (RT at 2.3, 2.7, 3.1, 3.3, 4.8, 6.1) confirmed that the complete degradation was occurred within 7 days. The isolate Aspergillus fumigatus were able to decolorize the textile synthetic dye, Acidic Blue - G. This research work reports that Aspergillus fumigatus has their potential for treatment of textile wastewaters before their discharge into water streams.