INVESTIGATION ON ANTIOXIDANT, ANTIMICROBIAL AND PHYTOCHEMICAL PROFILE OF BLUMEA LACERA LEAF
The present study we focuses on the phytochemical analysis, Antioxidant activity and Antimicrobial activity of Blumea lacera leaf collected from Akoli village, District Amravati (Central region of India). Phytochemical screening was carried out by ‘guide to modern techniques of plant analysisâ€. Furthermore antioxidant activity of methanolic extract of B.Lacera was investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antimicrobial activity of B.Lacera was investigated by Agar disc diffusion assay. The phytochemical screening of aqueous, methanolic and Chloroform leaf extracts revealed the presence of various secondary metabolites such as carbohydrates, alkaloids, phytosterols, steroids, phenolic compounds, tannins, flavonoids, cumarine, amino acids, terpenoids and saponins. The FTIR analysis of the crude extract of Blumea lacera gives information about the distribution of functional groups. DPPH free radical scavenging assay was studied for evaluation of antioxidant potential. The methanolic extract of Blumea lacera significantly inhibited the DPPH free radicals at the concentration ranging from 1000-5000 μg/ml. It shows the DPPH radical scavenging activity of Blumea lacera extract with ascorbic acid as reference, where the IC50 values for the Blumea lacera leaf extract (IC50= 2871 μg/ml) . The IC50 value were said to be less than the standard ascorbic acid (IC50= 2.816 μg/ml). The antimicrobial activity was done for ethyl acetate Blumea lacera leaf extracts. The study revealed that the leaf extracts of Blumea lacera showed highest antibacterial activity against S. aureus, P.auro, S.typhi. This study revealed the presence of phytochemical compounds and presence of functional groups. The study concluded that the methanolic extracts shows significant antioxidant activities in a concentration dependant manner and also shows significant antibacterial activity hence the plant contains potential antibacterial components that may be useful for evolution of pharmaceutical for the therapy of ailments.