A COMPARATIVE ASSESSMENT OF THE ANTIBACTERIAL EFFECTS OF Allium sativum AND ANTIBIOTICS ON NEW STRAINS OF Bacillus SPECIES ISOLATED FROM POULTRY FARM
The aim of this investigation was to evaluate the antibacterial activity of aqueous, ethanolic and methanolic extracts of Allium sativum at different concentrations against two new strains of bacteria (Bacillus subtilis strain AK and Bacillus licheniformis strain BIHPUR 0104) isolated from poultry farm by disc diffusion method. According to results obtained ethanolic extracts of Allium sativum had the best inhibitory activity showing maximum zone of 19.1 mm and 20 mm against B. subtilis strain AK and B.licheniformis strain BIHPUR 0104 respectively compared to aqueous and methanolic extracts. The aqueous extracts were found to be more effective compared to methanolic extracts against B.subtilis strain AK. But the methanolic extracts were showing more inhibitory action compared to aqueous extracts against B.licheniformis strain BIHPUR 0104. 100% extracts of Allium sativum were found to be more effective than Ampicillin against B.subtilis strain AK. On the other hand the extracts with the same concentration was less effective compared to Ampicillin and Cephotaxime against B.licheniformis strain BIHPUR 0104. Relative percentage inhibition of 100% Allium sativum extracts was calculated with respect to the antibiotics. 100% Allium sativum extracts showed the maximum relative percentage inhibition of 111.42% and 62.49% against B. subtilis strain AK and B.licheniformis strain BIHPUR 0104 with respect to Ampicillin and Cephotaxime respectively. The results of present study showed that Allium sativum can be used for the treatment of the infections caused by these two new strains of Bacillus species by inhibiting their growth