STUDY THE SUPPORTING EFFECT OF BIOEMULSIFIER EXTRACTED FROM SERRATIA LIQUEFACIENS TO SOME ANTIBIOTICS AGAINST PSEUDOMONAS AERUGINOSA
The aim of this study is use the safety biological material such as (bioemulsifier) to assist the chemical therapeutic material (antibiotics) in controlling the growth of multidrug resistant Pseudomonas aeruginosa. P. aeruginosa (P.) was isolated from different clinical specimens were obtained from Biology Department/ University of Baghdad; seven isolates of P. aeruginosa were selected and subjected to antibiotic sensitivity test for some antibiotic like: (cefatazidine(CAZ), chloramphenicol(C), nalidixic acid(NA), gentamycin(CN), amoxicillin(AMC), tetracycline(TE), tobramycin(TOB), ciprofloxacin(CIP), streptomycin(S), calarithromycin(CLR) and cefotaxime(CTX). Among various antibiotics tested most isolates showed highest resistance to TE, NA, S, CLR and CTX. So we used the crud bioemulsifier extracted from Serratia liquefaciens by using organic solvent diethyl ether (v:v) to support the mode of action of these antibiotics, the bioemulsifier was characterized by HPLC analysis to confirm the component of it which found it consist of fatty acids and carbohydrates, the combination of some antibiotics and bioemulsifier showed obviously increase of inhibition zones in some isolates in compare with the inhibition of antibiotics without it for ex: the inhibition of (C) for isolate P.3 was increase from 28mm to 36mm when used bioemulsifier in concentration(100mg/ml), also the P.7 exhibit resistance for TE without bioemulsifier and when used the bioemulsifier with TE the results were: 20mm and 12mm for the concentration(50&100mg/ml) respectively, so the conclusion is: P. aeruginosa isolates showed resistance for many antibiotics and when the bioemulsifier support the action of antibiotics that combination show an effect on some isolates growth.