BIO ANALYTICAL HPLC METHOD DEVELOPMENT AND VALIDATION OF ZOLMITRIPTAN IN RABBIT PLASMA
A simple, sensitive and rapid high performance liquid chromatographic method was developed and validated for Zolmitriptan from rabbit plasma. Chromatographic separation and detection was carried out on a Hibar C18 (250 x 4.6 mm, 5µ) coloum using 10 mM di-potassium hydrogen orthophosphate buffer (pH 3.2) and methanol in the ratio of 77:23 with a flow rate of 1.1 ml/min at 231 nm. Retention times of drug and IS were found out to be 5.5 min and 7.7 min respectively. The method was linear in the concentration range of 18.73-374.6 ng/ml. The regression coefficient value was found to be 0.987. The proposed method was validated by performing linearity, recovery, specificity, robustness, LOD/LOQ and interday / intraday precision. The LOD and LOQ values were found to be 6.18 and 18.73 ng/ml. Rizatriptan was used as internal standard