EVALUATION OF ANTIHYPER LIPEDEMIC ACTIVITY OF L CITRULLINE ON DIABETES INDUCED BY HYPER LIPEDEMIC RAT MODEL
Watermelon is rich source of L-citrulline, an effective precursor of L-arginine. This study was conducted to determine whether dietary supplementation with watermelon pomace juice could reduce hyperglycemia, hyperlipidemia and oxidative stress in alloxan induced diabetic rats. Nine albino rats prague dawely strain were used. The animals were divided into following groups as: Control group (N=3) and Diabetic group (N=6) which divided into two equal subgroups as following: untreated diabetic positive control group and treated diabetic group with 94.5% of watermelon juice for 30days. The beneficial role of watermelon juice was evaluated by orally administration to alloxan induced rats. Diabetic untreated control group showed significant reduction in body weight gain as compared to normal and treated groups, enhancement in Oxidative stress in rats as indicated by significant elevation of Malondialdehyde (MDA) level, significant elevation of serum TG, TC, LDL-C, VLDL-C levels. Whereas it was observed a significant reduction in liver glycogen content and serum HDL-C levels with respective negative control and treated groups. Oral treatment of diabetic rats with watermelon juice at 94.5% as indicates significant decrease p<0.05 in serum MDA, remarkable glycemic control, increase liver glycogen and improvement in lipid profiles, parameters as compared with diabetic control untreated group. Serum arginine was significantly increased in diabetic groups treated with 94.5% of watermelon juice as compared to diabetic untreated control. The present study demonstrates that, watermelon juice enhances arginine availability, increase serum arginine concentration and has a significant hypoglycemic, hypolipidamic effects and significantly modify the oxidative stress with the best effect obtained with administration of the high concentration watermelon juice 94.5% in diabetic rats