QUANTITATIVE DETERMINATION OF TANNIN CONTENT AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF CROSSANDRA INFUNDIBULIFORMIS (L) NEES AGAINST UTI PATHOGENS
The present study is designed to determine the tannin content and evaluation of antibacterial activity of Crossandra infundibuliformis (Acanthaceae) flower extracts against pathogens associated with urinary tract infections. The Urinary Tract Infections represent one of the most common diseases occurring from the neonate to the geriatric age groups encounters in medical practice today. The chloroform, ethyl acetate, acetone and aqueous extracts for the plant material were prepared. The extracts contained appreciable levels of tannin content (2.7 to 8.6 mg of GAE/gm extract). Among all the extracts acetone extract possess higher concentration of tannins. Leading etiological agents of UTI’s include Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus vulgaris. The susceptibility patterns of these UTI pathogens against extracts were tested. The antibacterial activity was prominent against Escherichia coli (16.20±0.25mm) by acetone extract. The results indicated that natural tannin from flowers of Crossandra infundibuliformis has a notable antibacterial activity against tested microorganisms. The results are compared with commercial antibiotic nitrofurantoin