PCL SCAFFOLD FOR NEURONAL PROGENITAL CELLS DERIVED FROM CORD BLOOD: TO RECONSTITUTE NERVE TISSUE
Neural progenital cells derived from human umbilical cord blood (HUCB-NPCs) is an ethically uncontroversial source of stem cells, able to differentiate into specific type of cells thats neuronal cells. The proliferative capacity of the HUCB-NPCs was monitored by the MTT test over fabricated PCL scaffold while their ability to differentiate into neural-like cells by Reverse Transcriptase (RT)-PCR. In preliminary experiments, human umbilical cord blood derived MNC cells cultured in vitro with various growth factors substantiate mesenchymal cell morphology, and were positive by RT-PCR for Nestin (neural),GAPDH (housekeeping gene), CD105 (endoglin), and CD90 (specific mesenchymal marker) markers and negative for Sox2 that indicates all mononuclear cells (MNCs) converted to NPCs. Neural progenitalcells cultured on the polycaprolactone (PCL) Scaffold i.e., PCL with polyethylene glycol(PEG) 4000 (5%,10%) & sucrose (5%,10%), fabricated by solvent casting & particulate leaching method, in differentiating medium over 21 days which exhibited a good confinement to the polypeptide domains of PCL. The number of cells attached increased linearly with the micro-film of PCL & PCL with Sucrose (5%, 10%). This article provides state-of-the-art on interactions between neural progenital cells derived from human cord blood and bioengineered surfaces