DNA DAMAGE IN LUNG CANCER PATIENTS AND CONTROL IRAQI SUBJECTS USING ELISA TECHNIQUE
Urinary 8- hydroxydeoxyguanosin (8-OHdG), oxidized form of the nucleoside 2’-deoxyguanosine present in DNA, is one of the most reliable and abundant markers of DNA damage. The present study was designed to evaluate the oxidative DNA damage in lung cancer patients and healthy negative control by using a urinary 8-OHdG as biomarker. Forty two urine samples of lung cancer patients (with new and advanced cancer identified in the Hospital of Radiotherapy and Nuclear Medicine/Baghdad) and healthy control subjects from September 2012 to April 2013 were investigated with ELISA technique (CUSABIO). The concentration of urinary 8-OHdG in lung cancer patients was significantly higher than in healthy negative control (637.08 ± 48.07 ng/ml versus 283.90 ± 43.14 ng/ml). As a conclusion in the present study, urinary 8-OHdG can be considered more helpful for the diagnosis of cancer since the tumor cells themselves produce radical oxygen species (ROS) spontaneously, which result in an increase of 8-OHdG in DNA and this technique is a useful tool for the diagnosis of cancer