SIMULTANEOUS ESTIMATION OF ANTI-OXIDANT FLAVONOIDS OF HIGH YIELD POLYPHENOLIC EXTRACT (HYPE) OBTAINED FROM AN AGRO WASTE MATERIAL OF P.GRANATUM BY HPLCDAD TECHNIQUE
Pomegranate is widely known to exhibit anti-viral, anti-oxidant, anti-cancer and anti-proliferative properties. The antioxidants like catechin, myricetin and quercetin in High Yield Polyphenolic Extract (HYPE) from agro waste of P.granatum with the aim of developing a rapid RP-HPLC method for detection of each and estimation of the anti-oxidants in HYPE extract. The samples were analysed using a HPLC system, standard solution and sample, a photo diode array detector, C18analytical column (4.6mm φ x 25. mm, 5µm particle size).The Chromatographic analysis of HYPE sample and standards were performed using gradient elusion with acetonitrile, water and phosphoric acid. Optimal detection of quantification was set to 272nm. Calibration exhibited good linearity R2 ≥ 0.999 ,accuracy and repeatability yielded good results of R.S.D <2.2% and recovery (85.7149-99.7385%).The extract of peel powder was found to contain catechin 115mg gm-1 , quercetin 18.3mg gm-1 and myricetin 1.5 mg gm-1 .The method was robust in detecting the three flavonoids within 12 minutes of elusion time with good resolution. Therefore we report a report an efficient and simultaneous estimation and detection of flavonoids in HYPE extract