BIOANALYTICAL METHOD FOR THE SIMULTANEOUS ESTIMATION OF RESVERATROL AND CURCUMIN IN RAT PLASMA
The present study was undertaken to develop and validate a bioanalytical method for simultaneous estimation of resveratrol and curcumin in rat plasma. A HPLC method with UV-visible detection was developed employing C-18 column. The mobile phase used was 10 mM ammonium acetate buffer and methanol. The developed method was validated as per the USFDA guidelines for bioanalytical method validation. The validation parameters are found to be well within the specifications. This method was also employed to estimate the plasma concentration of resveratrol and curcumin in the samples obtained from a single dose per oral pharmacokinetic study in Wistar rats and the plasma samples obtained from in vivo pharmacokinetic study in Wistar rats showed various extra peaks, which could be the metabolites of both the compounds, and the characterization of the extra peaks is underway. Thus the method developed and validated in the present study can be employed to estimate these two drugs simultaneously in all the in vivo studies, along with their metabolites