IN VITRO EFFECT OF RESVERATROL ON DNA METHYLATION AND BRCA1 EXPRESSION IN MCF-7 HUMAN BREAST CANCER CELLS
Research is focusing on the search for new types of natural chemotherapeutic agents derived from plants which are proving to be excellent sources of new compounds. Resveratrol is a natural polyphenolic compound, which is found in grapes, peanuts, berries, Polygonum root, known for its beneficial effects on energy homeostasis, and it also has multiple properties, including anti-oxidant, anti-inflammatory, and anti-tumor activities. The present study aims to investigate the effect of different concentrations of resveratrol on the expression of BRCA1 gene and level of DNA methylation in human breast carcinoma MCF-7 cells. Results revealed that resveratrol has cytotoxic activity against MCF-7 cells as compared to that of anticancer drug doxorubicin. Meanwhile, the expression of BRCA1 in MCF-7 cells lysate was significantly increased as compared to the untreated cells. Moreover, resveratrol significantly reduced the level of DNA methylation. These results indicate that resveratrol acts as a regulator for BRCA1 gene through the alteration of DNA methylation