SIMULTANEOUS ESTIMATION OF LAMIVUDINE, STAVUDINE AND NEVIRAPINE IN TABLET DOSAGE FORM BY RP-HPLC METHOD
A rapid RP-HPLC method was developed and validated for simultaneous estimation of Lamivudine, Stavudine and Nevirapine from pharmaceutical dosage forms. A sensitive chromatographic separation was accomplished on thermo scientific C18 column (100×4.6 mm, 5µ) with mobile phase consisting of Methanol: phosphate buffer (pH 4 adjusted with ortho phosphoric acid) in the ratio of 75:25 v/v, at a flow rate of 1.5 ml/min and eluents monitored at 268 nm. The developed method was validated in terms of accuracy, precision, linearity and limit of detection, limit of quantification, robustness and solution stability. The proposed method can be used for the estimation of these drugs in combined pharmaceutical dosage forms.