PHARMACOLOGICAL SCREENING OF POLY HERBAL FORMULATION FOR WOUND HEALING ACTIVITY ON ALBINO RATS
The present study was designed to evaluate wound healing activity of poly herbal formulation (Curcuma longa, Eclipta alba, and Tridax procumbens) in Wistar albino rats using Excision wound model. The back of each animal was shaved and prepared after washing with sprit. An area of about 2 cm was defined with marker on the shaven back of the animals. The circular marked area was excised with its full thickness using a surgical sterile blade and scissors under phenobarbitone anaesthesia.. Poly herbal ointment and SILVEREX -ointment were applied once daily for the 16 days starting from the day of wounding. The observations of percentage wound closure were made on 4th,8th,12th and 16th post wounding days. The wound area of each animal was measured at the intervals of 24-48 hrs using transparent polythene graph paper and then tracing the area of wound on it (Approx 500 sq.mm initial wound reading was observed. Contraction was calculated from the days of measurements of wound area. The result of this study strongly indicated that that group 2 produced greater wound contraction compared with the other tested groups and wound contraction increases as the day passes