PROPHYLACTIC STUDY IN MICE IMMUNIZED WITH LISTERIA MONOCYTOGENES ANTIGENS AND IMPLANTED WITH MAMMARY ADENOCARCINOMA (AM3)
Bioimmunotherapy was considered as another form of cancer therapy, which includes using of living microorganisms. To evaluate the host immune response by Listeria monocytogenes antigens (LMAgs) against tumor cells implantation. Eighty of female-white mice (5-6 weeks) were divided randomly into four equal groups, 1st group; immunized with 0.4ml (25mg/ml) of Culture-Filtrate LMAgs (CFLMAgs), 2nd; with Live-attenuated LM (LaLM) 0.4ml (1x109 CFU/ml), 3rd ; implanted with 2ml (10x107 cells/ml) of tumor cells (AM3) , 4th ; inoculated S/C with 0.4ml of sterile PBS. Cellular immunity was determined pre and post implantation.All animals sacrificed at four weeks post-tumor implantation. High mean values of IFN-γ (778.96±6.55) was observed in 2nd group and no tumor growth seen in immunized animals except small masses of tumor cells in the 1st group. Fibrous connective tissue proliferation with mononuclear cells infiltrate the site of implantation and small granulomatous lesions were seen in the internal organs of immunized-tumor implanted animals. LaLM and CFLM antigens provided good and partial immune responses, respectivly against tumor cells implantation