BIOFILM INHIBITORY EFFECT OF SILVER NANOPARTICLES COATED CATHETER AGAINST STAPHYLOCOCCUS AUREUS AND EVALUATION OF ITS SYNERGISTIC EFFECT WITH ANTIBIOTICS
The present study was undertaken to evaluate pharmacological inhibition of biofilm of clinical isolate of Staphylococcus aureus on silver nanoparticles coated catheter under in vitro condition and the synergistic effect of nanoparticles with antibiotics was studied. Silver nanoparticles synthesized by chemical reduction method were coated on the catheter adopting simple dispersion method. Initially biofilm inhibitory effect was studied with spectrophotometric method and the biochemical composition of biofilm matrix mainly total carbohydrates and protein studied. Silver nanoparticles synthesized by chemical reduction of silver nitrate with sodium borohydride were coated on the catheter was characterized by surface topography of catheter by scanning electron microscopy which reveals complete dispersion of the nanoparticles on the fibre surface of the catheter and the size, shape of the particles shows uniform spherical particles with the size of 40-60 nm. Distinct effect of biofilm inhibition was recorded in the nanoparticles coated catheter and maximum inhibition was observed during 24th hour of incubation Surface topography of nanoparticles and nanoparticles with antibiotics coated catheter with scanning electron microscopy reveals the complete degeneration of the biofilm whereas biofilm matrix biochemical composition mainly total carbohydrates and total protein was highly reduced. Biofilm inhibition rate and reduction of biofilm matrix biochemical composition was increased in nanoparticles with all the tested antibiotics treatment which suggests the synergistic effect.