657NEPHROTOXIC EFFECT OF ETHANOLIC EXTRACT OF SYZYGIUM CUMINI. LINN LEAVES ON EXPERIMENTAL ANIMALSSyzygium cumini. Linn Leaves were broadly used in treatment of several diseases like antihypertensive, carcinomas, microbial infections etc. The intention of our study was to investigate the acute and sub-chronic oral toxicities on Ethanolic Extract of Syzygium cumini. Linn Leaves in experimental rats. Acute toxicity study was carried out using the fixed dose method according to OECD guideline no. 423. The different doses like 500, 1000, 2000, 3000, 4000 and 5000mg/Kg body weight were administered orally to the animals, observed for 24 hr after dosing and also observed for 14 days without giving drug. In subchronic oral toxicity study, evaluations were carried out after administering daily oral doses of 1250, 2500 and 5000 mg/kg body weight for 28 days to the rats. Body weights of the rats observed weekly and Biochemical, hematological, histopathological assessments and relative organ weights of the rats were observed on 29th day. Acute oral administration of Ethanolic extract of Syzygium cumini leaves to experimental rats at a dose level of up to 5000mg/kg did not cause any mortality or toxic symptoms but in subchronic repeated oral administration caused significant increase in kidney size, Hematological parameters (RBC, WBC & Hb) and Increase urea and creatinine levels in group II, III and IV compare to group-I. By observing the hematological, biochemical parameters and the histopathological studies it is finally concluded that Ethanolic extract of Syzygium cumini leaves produces Multifocal Moderate tubular nephritis, multifocal moderate tubular degeneration in the kidney at oral doses of 1250, 2500 and 5000mg/kg body weightcayyanna5@gmail.comResearch682015Syzygium cumini. Linn Leaves, Acute and Sub Chronic toxicity,EESCL,Hematology,HistopathologyChakali Ayyanna,Munuswamy Sekar, Raghupathi Niranjan kumar,Poojari Veera Narendra,Vennapusa Narendranath Reddy Department of Pharmacology, Santhiram College of Pharmacy, Nandyal, Andhra Pradesh, India,Department of Pharmaceutical Chemistry, Santhiram College of Pharmacy, Nandyal, Andhra Pradesh, India,Department of Pharmacology, Santhiram College of Pharmacy, Nandyal, Andhra Pradesh, India, Santhiram College of Pharmacy, Nandyal, Andhra Pradesh, India., Santhiram College of Pharmacy, Nandyal, Andhra Pradesh, India.